Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis
Identifieur interne : 001147 ( Main/Exploration ); précédent : 001146; suivant : 001148Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis
Auteurs : Kathryn M. Meurs [États-Unis] ; Philip R. Fox [États-Unis] ; Alexander L. Magnon [États-Unis] ; Si-Kwang Liu [États-Unis] ; Jeffrey A. Towbin [États-Unis]Source :
- Cardiovascular Pathology [ 1054-8807 ] ; 2000.
English descriptors
- Teeft :
- Animal model, Arrhythmogenic right, Calici, Calici virus, Canine, Canine parvovirus, Cardiac, Cardiomyopathy, Cardiovasc, Cardiovasc pathol, Cell cultures, Coll cardiol, Control cats, Corona, Corona virus, Endomyocardial biopsies, Etiology, Feline, Feline cardiomyopathy, Feline panleukopenia, Feline panleukopenia virus, Genome, Heart failure, Herpes, Herpes virus, Histopathologic, Histopathologic evidence, Human disease, Hypertrophic, Hypertrophic cardiomyopathy, Idiopathic, Idiopathic cardiomyopathy, Inflammation, Light microscopy, Meurs, Molecular detection, Myocardial, Myocardial tissue, Myocarditis, Myocarditis years, Myocardium, Nonstructural protein, Panleukopenia, Panleukopenia virus, Panleukopenia virus genome, Parvovirus, Pathogenesis, Pathol, Polymerase, Polymerase chain reaction, Positive control, Primer, Restrictive cardiomyopathy, Sudden death, Towbin, Ventricular, Viral, Viral genome, Viral infection, Viral myocarditis.
Abstract
Abstract: Viral myocarditis has been suggested as an etiology for cardiomyopathy in several mammalian species. Myocarditis and idiopathic cardiomyopathy have been reported in the domestic cat, although a viral etiology has not been demonstrated. Because of the continuing interest in the potential relationship between viral myocarditis and cardiomyopathy, we evaluated hearts from cats with spontaneous, idiopathic cardiomyopathy for viral genomic material within myocytes by polymerase chain reaction, and for the presence of myocarditis by light microscopy. Thirty-one (31) formalin-fixed hearts from domestic cats who died of idiopathic cardiomyopathy were randomly selected from pathology archives. Seventeen (17) formalin-fixed hearts from healthy cats were similarly selected as normal controls. The polymerase chain reaction (PCR) was used to evaluate myocardial tissue for the presence of viral genome from feline panleukopenia virus, herpes virus, calici virus, and corona virus. Hearts were examined using light microscopy for histologic evidence of myocarditis according to the Dallas criteria. Panleukopenia virus was identified by PCR in 10 of 31 cats with cardiomyopathy but in none of the controls. Neither cardiomyopathic or control cats tested positive by PCR for herpes virus, calici virus, and corona virus. Myocarditis was detected by histologic examination in 18 of 31 cardiomyopathic cats and in none of 17 control cats. Myocarditis and or feline panleukopenia virus genome was detected in felines with idiopathic hypertrophic, dilated, and restrictive cardiomyopathy, suggesting a possible role of viral infection and inflammation in the pathogenesis of cardiomyopathy in this species.
Url:
DOI: 10.1016/S1054-8807(00)00031-4
Affiliations:
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Towbin</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Baylor College of Medicine, Houston, TX</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Cardiovascular Pathology</title><title level="j" type="abbrev">CVP</title><idno type="ISSN">1054-8807</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">9</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="119">119</biblScope><biblScope unit="page" to="126">126</biblScope></imprint><idno type="ISSN">1054-8807</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1054-8807</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Animal model</term><term>Arrhythmogenic right</term><term>Calici</term><term>Calici virus</term><term>Canine</term><term>Canine parvovirus</term><term>Cardiac</term><term>Cardiomyopathy</term><term>Cardiovasc</term><term>Cardiovasc pathol</term><term>Cell cultures</term><term>Coll cardiol</term><term>Control cats</term><term>Corona</term><term>Corona virus</term><term>Endomyocardial biopsies</term><term>Etiology</term><term>Feline</term><term>Feline cardiomyopathy</term><term>Feline panleukopenia</term><term>Feline panleukopenia virus</term><term>Genome</term><term>Heart failure</term><term>Herpes</term><term>Herpes virus</term><term>Histopathologic</term><term>Histopathologic evidence</term><term>Human disease</term><term>Hypertrophic</term><term>Hypertrophic cardiomyopathy</term><term>Idiopathic</term><term>Idiopathic cardiomyopathy</term><term>Inflammation</term><term>Light microscopy</term><term>Meurs</term><term>Molecular detection</term><term>Myocardial</term><term>Myocardial tissue</term><term>Myocarditis</term><term>Myocarditis years</term><term>Myocardium</term><term>Nonstructural protein</term><term>Panleukopenia</term><term>Panleukopenia virus</term><term>Panleukopenia virus genome</term><term>Parvovirus</term><term>Pathogenesis</term><term>Pathol</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Positive control</term><term>Primer</term><term>Restrictive cardiomyopathy</term><term>Sudden death</term><term>Towbin</term><term>Ventricular</term><term>Viral</term><term>Viral genome</term><term>Viral infection</term><term>Viral myocarditis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Viral myocarditis has been suggested as an etiology for cardiomyopathy in several mammalian species. Myocarditis and idiopathic cardiomyopathy have been reported in the domestic cat, although a viral etiology has not been demonstrated. Because of the continuing interest in the potential relationship between viral myocarditis and cardiomyopathy, we evaluated hearts from cats with spontaneous, idiopathic cardiomyopathy for viral genomic material within myocytes by polymerase chain reaction, and for the presence of myocarditis by light microscopy. Thirty-one (31) formalin-fixed hearts from domestic cats who died of idiopathic cardiomyopathy were randomly selected from pathology archives. Seventeen (17) formalin-fixed hearts from healthy cats were similarly selected as normal controls. The polymerase chain reaction (PCR) was used to evaluate myocardial tissue for the presence of viral genome from feline panleukopenia virus, herpes virus, calici virus, and corona virus. Hearts were examined using light microscopy for histologic evidence of myocarditis according to the Dallas criteria. Panleukopenia virus was identified by PCR in 10 of 31 cats with cardiomyopathy but in none of the controls. Neither cardiomyopathic or control cats tested positive by PCR for herpes virus, calici virus, and corona virus. Myocarditis was detected by histologic examination in 18 of 31 cardiomyopathic cats and in none of 17 control cats. Myocarditis and or feline panleukopenia virus genome was detected in felines with idiopathic hypertrophic, dilated, and restrictive cardiomyopathy, suggesting a possible role of viral infection and inflammation in the pathogenesis of cardiomyopathy in this species.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Ohio</li><li>Texas</li><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="Ohio"><name sortKey="Meurs, Kathryn M" sort="Meurs, Kathryn M" uniqKey="Meurs K" first="Kathryn M" last="Meurs">Kathryn M. Meurs</name></region><name sortKey="Fox, Philip R" sort="Fox, Philip R" uniqKey="Fox P" first="Philip R" last="Fox">Philip R. Fox</name><name sortKey="Liu, Si Kwang" sort="Liu, Si Kwang" uniqKey="Liu S" first="Si-Kwang" last="Liu">Si-Kwang Liu</name><name sortKey="Magnon, Alexander L" sort="Magnon, Alexander L" uniqKey="Magnon A" first="Alexander L" last="Magnon">Alexander L. Magnon</name><name sortKey="Meurs, Kathryn M" sort="Meurs, Kathryn M" uniqKey="Meurs K" first="Kathryn M" last="Meurs">Kathryn M. Meurs</name><name sortKey="Towbin, Jeffrey A" sort="Towbin, Jeffrey A" uniqKey="Towbin J" first="Jeffrey A" last="Towbin">Jeffrey A. Towbin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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